Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments     

Gozari Mohsen  Bahador Nim  Jassbi Amir Rez  Mortazavi Mohammad Seddiq  Hamzehei Sam  Eftekhar Ebrahim 《海洋学报(英文版)》2019,38(12):84-90

Screening bioactive natural products from bacteria is a determinative step in the drug discovery programs. The present study aim to isolate actinobacteria from the Oman Sea sediments for determining the effects of different culture media and treatments on the yield of the isolation process, and measure the DPPH radical scavenging and Artemia cytotoxic activity of culture extracts of the actinobacterial isolates. A total of 290 actinobacterial isolates were collected from 14 sediment samples. Heat treatment(40.68%) and M_4 medium(29.31%) exhibited the maximum isolation rates of actinobacteria. Streptomyces isolates were dominantly distributed in all of the investigated stations according to 16 S rRNA gene sequencing. The distribution pattern of Streptomyces followed a depth-dependent frequency trend, whereas the members of rare genera including Micromonospora, Nocardia Actinoplanes, Nocardiopsis, Saccharopolyspora and Crossiella were distributed in deeper stations. Approximately,25% of the examined isolates could scavenge 90% of 10~(–4) mol/L DPPH solutions at 1 250 μg/mL final concentration of their ethyl acetate culture extracts. Furthermore, the most potent extracts could scavenge DPPH radicals with IC50 ranges from 356.8 to 566.4 μg/mL. Brine shrimp cytotoxicity tests showed that 38.88% of the examined culture extracts exhibited LC_(50) lower than 1 000 μg/mL against the Artemia cells. Moreover, the most potent culture extracts exhibited LC_(50) range from 335.4 to 534.4 μg/mL. Phylogenetic analysis by 16 S rRNA gene sequence revealed that the OS 005, OS 263 and OS 157 closely related to Streptomyces djakartensis, Streptomyces olivaceus and Nocardiopsis dassonvillei respectively. These results suggested the widespread distribution of the antioxidant and cytotoxic producing actinobacteria in the Oman Sea sediments, which could be considered as promising candidates for the discovery of microbial bioactive compounds.  相似文献   

中国主要入海河口咸潮入侵变化特征     

李文善  左常圣  王慧  董军兴  高通  潘嵩  金波文 《海洋通报》2019,38(6):650-655

长江口、钱塘江口和珠江口是受咸潮影响较为严重的区域。本文利用全国沿海海平面变化影响调查、沿海水文观测等数据,分析了近十年长江口、珠江口和钱塘江口咸潮入侵的变化特征及影响。分析结果表明:(1) 2009-2018年,长江口咸潮入侵次数和持续时间均呈减少趋势,该时段长江口共监测到约48次咸潮入侵过程,发生时间集中在9-10月至翌年5月,其中3月和11月入侵次数较多,分别为12次和7次。(2)钱塘江口咸潮入侵过程受沿海季节性海平面影响显著,12月至翌年3月为钱塘江口季节性低海平面期,4-7月上旬径流量较大,上述两个时期钱塘江口受咸潮入侵的影响均较小,7月下旬至11月上旬,钱塘江口处于季节性高海平面期,是咸潮影响的集中时段。(3) 2009-2018年,珠江口共监测到约57次咸潮入侵过程,发生时间集中在9-10月至翌年3-4月,其中1月、2月和10月咸潮入侵次数较多,均超过10次,2015年至今咸潮持续时间明显增加。(4)咸潮入侵次数和持续时间与基础海面和径流量等密切相关,咸潮入侵影响三大河口沿线水厂供水以及工农业生产取水,给沿岸城市的居民生活、工农业生产和渔业养殖等造成一定不利影响。  相似文献   

Conversion relationship of rainfall-soil moisture-groundwater in Quaternary thick cohesive soil in Jianghan Plain,Hubei Province,China     

《China Geology》2020,3(3):462-472

The scientific field test site of rainfall-soil moisture-groundwater conversion in Dabie Mountain Area–Jianghan Plain is located in the northern region of the Jianghan Plain, the transition zone between the Dabie Mountain Area and Jianghan Plain. It’s a great field test site to study the material and energy exchange among rainfall, soil moisture, and groundwater of the Earth ’s critical zone in subtropical monsoon climate plain areas. This paper analyzed the connection between rainfall and volume water content (VWC) of soil at different depths of several soil profiles, and the dynamic feature of groundwater was discussed, which reveals the rainfall infiltration recharge of Quaternary Upper Pleistocene strata. The results show that the Quaternary Upper Pleistocene aquifer groundwater accepts a little direct rainfall recharge, while the lateral recharge is the main supplement source. There were 75 effective rainfall events among 120 rainfall events during the monitoring period, with an accumulated amount of 672.9 mm, and the percentages of effective rainfall amount and duration time were 62.50% and 91.56%, respectively. The max evaporation depth at the upper part in Quaternary cohesive soil was no less than 1.4 m. The soil profile was divided into four zones: (1) The sensitive zone of rainfall infiltration within 1.4 m, where the material and energy exchange frequently near the interface between atmosphere and soil; (2) the buffer zone of rainfall infiltration between 1.4 m and 3.5 m; (3) the migration zone of rainfall infiltration between 3.5 m and 5.0 m; and (4) the rainfall infiltration and groundwater level co-influenced zone below 5.0 m. The results revealed the reaction of soil moisture and groundwater to rainfall in the area covered by cohesive soil under humid climate in Earth ’s critical zone, which is of great theoretical and practical significance for groundwater resources evaluation and development, groundwater environmental protection, ecological environmental improvement, drought disaster prevention, and flood disaster prevention in subtropical monsoon climate plain areas.  相似文献   

Geochemical characteristics and sedimentary environment of the Middle Devonian organic-rich shales in the Northwest of Guizhong Depression,Southwest China     

《China Geology》2020,3(4):567-574

In order to figure out the redox conditions and paleo-sedimentary environment of the Middle Devonian shales in the northwest of Guizhong Depression, the trace element analysis was conducted on the Middle Devonian cores (320.35–938.50 m) of the typical shale gas investigation well (GY-1) at a 1.50 m sampling interval through X-ray fluorescence spectroscopy (XRF) and inductively coupled plasma mass spectrometry (ICP-MS). According to the test result, the average values of V/(V+Ni), V/Cr and Ni/Co in Nabiao formation (Fm.) are larger than 0.67, 4.65 and 7.71 respectively, and Nabiao Fm. is rich in biological assemblages such as tabasheer, ammonite, etc. These evidences indicate the rising sea level rose relatively in the sedimentation period of Nabiao Fm. and a deepwater shelf environment, which was favorable for the preservation of organic matters. The V/(V+Ni), V/Cr and Ni/Co in Luofu Fm. and Tangting Fm. are 0.38–0.65, 0.73–4.10 and 3.70–6.72 respectively, indicating that the sea level dropped relatively in their sedimentation period, during which the water bodies became shallow, and the sedimentary environment was a weak oxidizing shallow water shelf environment. In addition, the variation of TOC has a high correlation with the enrichment degree of Ba element, indicating the favorable conditions for the enrichment and preservation of organic matters under an oxygen-deficient environment. Moreover, according to the identification of trace element indexes, the northwest of Guizhong Depression experienced the sedimentary cycle of relative rise to relative fall of sea level from bottom to top in the Middle Devonian sedimentation period. The relative sea level rose to the highest in the sedimentation period of Nabiao Fm., in which the organic-rich shales with stable thickness and high organic content were deposited. Hence, the Nabiao Fm. could be regarded as the favorable exploration target interval in this area.  相似文献   

载人潜水器运行管理机制探析          下载免费PDF全文

齐海滨  李德威  刘保华  杨雷  刘晓辉 《海洋开发与管理》2019,36(7):3-7

为了探索深海,不同类型的深海运载装备得以成功开发,并应用于深海科学、资源勘探开发、工程保障及战略安全等不同领域。深海运载装备可以分为载人潜水器(HOV)、遥控深潜器(ROV)、自治式深潜器(AUV)及混合型深潜器,其中以载人潜水器为代表的高技术装备群成为建设海洋强国的重要保障力量。文章对美国"阿尔文"号、俄罗斯"和平I"号和"和平II"号、日本"深海6500"号、法国"鹦鹉螺"号、中国"蛟龙"号载人潜水器运行管理机制进行了阐述,综合比较其优缺点,提出了建立职业化运行保障队伍、优化作业流程,创新组织结构、探索多型潜水器海上作业模式等运行管理机制构架的建议。  相似文献   

北半球热带中太平洋与印度洋海表温度梯度对夏季西北太平洋热带气旋生成频数变化的影响     

方珂  余锦华 《热带海洋学报》2019,38(5):42-51

基于1951—2018年哈德里中心海温资料、美国气象环境预报中心和美国国家大气研究中心再分析资料和第四代欧洲中心汉堡模式, 针对1994年、2018年等西北太平洋热带气旋(TC)生成异常多的年份, 研究了引起TC增加的海表温度异常(SSTA)模态及其影响机制。结果表明, 北半球热带中太平洋增暖与印度洋变冷是夏季西北太平洋TC生成频数增加的主要原因, 北大西洋负三极型式SSTA促使TC生成的进一步增加。热带中太平洋增暖与印度洋冷却在菲律宾以东激发出西风异常和气旋性环流异常。北大西洋负三极型式SSTA在我国南海、菲律宾至东南沿岸激发出气旋性环流异常。前者在西北太平洋中部, 后者在南海产生有利于TC生成的局地环境。1994年和2018年夏季热带中太平洋出现暖SSTA、印度洋为冷SSTA、北大西洋呈现负三极型式SSTA, 西北太平洋TC生成频数极端增多。近30年来, 当出现热带中太平洋增暖和印度洋冷却时, 北大西洋表现出比1989年以前更强的负三极型式SSTA, 使西北太平洋TC生成频数和北半球热带印度洋-太平洋SSTA梯度的线性相关更显著。  相似文献   

中国物理海洋学研究70年:发展历程、学术成就概览   总被引：2，自引：2，他引：0  

魏泽勋  郑全安  杨永增  刘克修  徐腾飞  王凡  胡石建  谢玲玲  李元龙  杜岩  周磊  林霄沛  胡建宇  朱建荣  李均益  张正光  侯一筠  刘泽  田纪伟  黄晓冬  管玉平  刘志宇  杨庆轩  赵玮  宋振亚  刘海龙  董昌明  于卫东  连涛  陈朝晖  史久新  雷瑞波  刘煜  于福江  尹宝树  陈戈  王岩峰  李整林  熊学军  汪嘉宁  李晓峰  王永刚 《海洋学报》2019,41(10):23-64

本文概略评述新中国成立70年来物理海洋学各分支研究领域的发展历程和若干学术成就。中国物理海洋学研究起步于海浪、潮汐、近海环流与水团，以及以风暴潮为主的海洋气象灾害的研究。随着国力的增强，研究领域不断拓展，涌现了大量具有广泛影响力的研究成果，其中包括:提出了被国际广泛采用的“普遍风浪谱”和“涌浪谱”，发展了第三代海浪数值模式；提出了“准调和分析方法”和“潮汐潮流永久预报”等潮汐潮流的分析和预报方法；发现并命名了“棉兰老潜流”，揭示了东海黑潮的多核结构及其多尺度变异机理等，系统描述了太平洋西边界流系；提出了印度尼西亚贯穿流的南海分支(或称南海贯穿流)；不断完善了中国近海陆架环流系统，在南海环流、黑潮及其分支、台湾暖流、闽浙沿岸流、黄海冷水团环流、黄海暖流、渤海环流，以及陆架波方面均取得了深刻的认识；从大气桥和海洋桥两个方面对太平洋–印度洋–大西洋洋际相互作用进行了系统的总结；发展了浅海水团的研究方法，基本摸清了中国近海水团的分布和消长特征与机制，在大洋和极地水团分布及运动研究方面也做出了重要贡献；阐明了南海中尺度涡的宏观特征和生成机制，揭示了中尺度涡的三维结构，定量评估了其全球物质与能量输运能力；基本摸清了中国近海海洋锋的空间分布和季节变化特征，提出了地形、正压不稳定和斜压不稳定等锋面动力学机制；构建了“南海内波潜标观测网”，实现了对内波生成–演变–消亡全过程机理的系统认识；发展了湍流的剪切不稳定理论，提出了海流“边缘不稳定”的概念，开发了海洋湍流模式，提出了湍流混合参数化的新方法等；在海洋内部混合机制和能量来源方面取得了新的认识，并阐述了混合对海洋深层环流、营养物质输运等过程的影响；研发了全球浪–潮–流耦合模式，推出一系列海洋与气候模式；发展了可同化主要海洋观测数据的海洋数据同化系统和用于ENSO预报的耦合同化系统；建立了达到国际水准的非地转(水槽/水池)和地转(旋转平台)物理模 型实验平台；发展了ENSO预报的误差分析方法，建立了海洋和气候系统年代际变化的理论体系，揭示了中深层海洋对全球气候变化的响应；初步建成了中国近海海洋观测网；持续开展南北极调查研究；建立了台风、风暴潮、巨浪和海啸的业务化预报系统，为中国气象减灾提供保障；突破了国外的海洋技术封锁，研发了万米水深的深水水听器和海洋光学特性系列测量仪器；建立了溢油、危险化学品漂移扩散等预测模型，为伴随海洋资源开发所带来的风险事故的应急处理和预警预报提供科学支撑。文中引用的大量学术成果文献(每位第一作者优选不超过3篇)显示，经过70年的发展，中国物理海洋学研究培养了一支实力雄厚的科研队伍，这是最宝贵的成果。这支队伍必将成为中国物理海洋学研究攀登新高峰的主力军。  相似文献   

黄河三角洲孤东近岸冲淤演变及其影响因素   总被引：1，自引：0，他引：1  

程慧  陈沈良  徐丛亮  姬泓宇  凡姚申 《海洋科学》2019,43(11):11-18

为更好地了解近40年来孤东近岸的演变过程,以研究区剖面水深地形、Landsat影像和利津站水沙数据为数据源,采用遥感技术及数理统计法对研究区域岸线及面积变化进行监测计算,并分析1976—1986年、1986—1996年、1996—2002年、2002—2014年4个不同阶段的冲淤演变及影响因素。结果表明:(1)孤东近岸经历"强淤积-冲淤平衡-侵蚀-强侵蚀"4个阶段。孤东近岸海域由淤积向侵蚀转变始于1996年,且在2002—2014年间侵蚀最为严重,大部分近岸海域蚀深达到6～8 m,侵蚀最大深度超过8 m;(2)等深线变化时空差异明显,蚀退最先出现在北侧,且近岸5 m水深区域内冲淤变化较水深10 m内敏感;(3)研究区近岸侵蚀,离岸淤积,剖面冲淤平衡位置由CS19剖面的11 m水深变化到CS21剖面的5m水深;(4)黄河入海水沙的减少、河口人工改汊、孤东大堤建设和海洋动力作用都对孤东近岸的冲淤演变产生影响,维持研究区冲淤平衡的年均来沙阈值为3.78亿t/a。通过此来进一步探究孤东近岸演变进程,为孤东近岸防护提供科学指导。  相似文献   

尼日尔三角洲东部深水区构造特征及其形成机理     

赵江  王秋良  卢圣力  付博  杜峰 《大地测量与地球动力学》2019,39(7):691-695

研究尼日尔三角洲东部深水区块发现，整个盆地从陆向洋具有3个大的构造分区：伸展拉张区、过渡区和挤压逆冲区。伸展区以大型同沉积断层伴生大量滚动背斜构造为特征，过渡区发育大量泥底辟构造，挤压区以复杂的逆冲叠瓦构造为主。通过分析形成机理，揭示东部深水转换带上M研究区构造特征，按构造的演化特征将该区构造分为泥底辟型、冲断-泥底辟混合型、逆冲型3种类型，提出研究区内的圈闭主要以构造-岩性圈闭为主，为尼日尔三角洲盆地深水勘探提供新的理论指导。  相似文献   

Dynamic loading tests and seismic response analysis of a stud-type damper composed of multiple friction units with disc springs     

Takeshi Sano  Kazutaka Shirai  Yasumasa Suzui  Yoshikazu Utsumi 《地震工程与结构动力学》2020,49(13):1259-1280

This paper presents an experimental study on the performance of a shear-sliding stud-type damper composed of multiple friction units with high-tension bolts and disc springs. A numerical evaluation of the response reduction effects achieved by the stud-type damper is also presented. In dynamic loading tests, the behavior of stud-type multiunit friction damper specimens was investigated. Three different full-scale damper specimens, which were composed of five, six, or seven friction units with two or four sliding surfaces, were incorporated into loading devices for testing. The stud-type friction dampers demonstrated stable rigid-plastic hysteresis loops without any remarkable decrease in the sliding force even when subjected to repetitive loading, in addition to showing no unstable behavior such as lateral buckling. The damper produced a total sliding force approximately proportional to the number of sliding surfaces and friction units. The total sliding force of the stud-type damper can thus be estimated by summing the contributions of each friction unit. In an earthquake response simulation, the control effects achieved by stud-type dampers incorporated into an analytical high-rise building model under various input waves, including long-period, long-duration and pulse-like ground motions, were evaluated. A satisfactory response reduction was obtained by installing the developed stud-type dampers into the main frame without negatively impacting usability and convenience in terms of building planning.  相似文献